↓研究　↓業績：英語論文（2010～）

基礎研究・臨床研究の紹介

骨肉腫細胞由来の細胞外小胞はmiR-146a-5pを介して破骨細胞の分化を抑制し遠隔転移を促進する

Front Oncol 2021

骨肉腫は小児から思春期・若年成人に多く発症し、診断時に肺などに遠隔転移を有する患者の5年生存率は約10-20％と予後不良である。より良好な治療成績を目指し、標準化学療法にゾレドロン酸の併用療法を行ったフランスと中国の第III相臨床試験において、併用療法群の方がより予後不良との結果が示された。このことから破骨細胞の分化を抑制することは予後不良となることが推測されたが、その機序に関しては不明とされた。そこで、近年注目されている、様々な細胞が細胞間情報伝達のために分泌している細胞外小胞（30-150nmの小さなものは特にエクソソームと呼ばれる）、特に骨肉腫細胞由来の細胞外小胞が、破骨細胞の分化とその予後にどのような機序で関与しているかを解明することを研究の目的とした。まず、細胞外小胞の分泌を抑制するTSG101遺伝子をノックアウトした骨肉腫細胞株を樹立し、マウスの下肢骨への移植実験で野生株と比較したところ、Ki-67index、CD31、VEGF、肺転移巣の数及び面積は有意に減少したが、Cathepsin K陽性の破骨細胞は保持されていた。続いて、マウス骨髄細胞を用いた破骨細胞分化誘導実験において、骨肉腫細胞由来の細胞外小胞を添加した群で有意に分化した破骨細胞の数が減少することが確認された。また培養細胞上清のqRT-PCRの結果から、骨肉腫細胞由来の細胞外小胞添加群においてNfatc1、Atp6v0d2、Dcstamp、OcstampはmRNAレベルで有意に減少した。さらに培養細胞上清のWestern Blotの結果からNF-κB及びIκBαのリン酸化がタンパクレベルで抑制されていることが確認された。次に悪性度の異なる2種類のヒト骨肉腫細胞（HOS、143B）の細胞外小胞に含まれるmiRNAをマイクロアレイによる全網羅的解析を行い、高悪性度細胞株である143Bに5倍以上含まれる16種類のmiRNAを同定し、その中から破骨細胞分化カスケードにおいてNF-κBの上流にあるTRAF6を標的とするmiR-146a-5pを特定した。miR-146a-5pを遺伝子導入した骨肉腫細胞株を作成し、破骨細胞分化誘導実験を行ったところ、細胞培養上清のqRT-PCR及びWestern BlotでTRAF6の産生が減少し、組織で破骨細胞の分化が有意に抑制されていることを確認した。最後に骨肉腫細胞の治療前の生検組織内の分化した破骨細胞数により2群に分け、その予後を比較したところ、分化した破骨細胞数が多いほど有意に予後が良好であることが示された。以上のことから、骨肉腫細胞由来の細胞外小胞内分子の一つであるmiR-146a-5pが、前破骨細胞内のTRAF6の転写産物を分解することで、破骨細胞の分化の抑制及び、肺への遠隔転移を促進し、骨肉腫患者の予後を不良にするという一機序を見いだした。

骨欠損への使用を目的としたヨード含有リン酸カルシウムセメントの抗菌効果と生体反応

J Biomater Appl 2023

リン酸カルシウムセメント(CPC)は, 骨腫瘍や骨折の治療後に発生する骨欠損の修復に多く使用される. 感染リスクの高い骨欠損症例に対応するためには, 抗菌スペクトラムが広く, 抗菌効果が長期間持続するCPCの開発が必要である. ポビドンヨードは広い抗菌スペクトルを有しており, 本研究ではポビドンヨードを含有したCPCの抗菌効果および生体反応について検討した. 5 %ヨード濃度のCPCは1週時以降に他のCPCよりもヨードを多く保持しており, 8週時まで黄色ブドウ球菌(ATCC 25923), 大腸菌(ATCC 700926)に対して抗菌効果を有していた. また, 5％ヨード濃度のCPCの抽出液は0％ヨード濃度のCPCおよびコントロール群の抽出液と同様のV79 cellのコロニー形成を認め, 細胞毒性が少ないことを示した. ウサギの大腿骨に挿入した5%ヨード濃度のCPCは, 0％および20％ヨード濃度のCPCと同様の骨形成を認め, 骨伝導能を抑制しなかった.

以上より, 5％ヨード濃度のCPCは将来, 感染リスクの高い骨欠損症例に適用されることが期待される.

液体窒素処理担癌神経グラフトを用いた新しい末梢神経再建法

Clinical Orthopaedic and Related Research 2022

神経に近接する軟部肉腫では，神経の合併切除に伴う神経脱落症状が問題となる．近年，軟部肉腫においては患肢温存手術が主流となり，腫瘍切除後の一期的な末梢神経再建の有用性が示されてきている．しかし，依然として神経再建のゴールドスタンダードは自家神経移植であることから，神経採取に伴う障害や，再建距離の限界といった問題がある．本研究においては自家神経移植のように採取部の障害がなく，さらに切除した長さと等長の神経グラフトを得ることができる液体窒素処理担癌神経グラフトの有用性と安全性をin vivoで評価した．F344/NJcl-rnu/rnuヌードラットの坐骨神経周囲に，ヒト線維肉腫（HT1080）株を移植して担癌神経を作成した。液体窒素処理担癌神経グラフト移植モデルにおいては、病理組織学的評価では，神経再生所見が見られ，48週モデルにおいても腫瘍の再発は認めなかった．液体窒素処理担癌神経グラフトは，将来的に悪性軟部肉腫術後患者のより良い患肢機能再建の一助となる可能性がある．

内側型変形性膝関節症に対するFocal Dome Condylar Osteotomy(FDCO)

Arthrosc Tech 2020

内反型変形性膝関節症に対する高位脛骨骨切り術(HTO)は内固定材料の改良と2面骨切りによる骨癒合促進効果により有用な治療法として広く認識されるに至っている．しかしながら，脛骨粗面より近位での骨切りは術後に相対的な膝蓋骨低位を生じ，膝蓋大腿関節圧の増加を来すことで経時的に関節症性変化を来すリスクが指摘されるようになった．また，変形矯正の理論体系から考えると，変形中心(CORA)から離れた部位で矯正を行うことは近接する足関節が非生理的なアライメントとなる危険性がある．

これに対しわれわれは，「関節不安定性の制動」，「生理的な足関節アライメントの再建」，「膝蓋骨低位の予防」，「広い骨接触面積による骨癒合促進」を得ることを目的にCORAを中心とした粗面下ドーム状骨切りと関節内骨切りを組み合わせるFocal Dome Condylar Osteotomy(FDCO)を開発した．

手術模式図

模擬骨を用いた手術の手順

臨床例，術前と術後

軟部腫瘍に対する新規分子標的薬オララツマブとAI療法の併用療法の可能性－患者由来組織同所移植モデルを用いた網羅的解析－

Cancer Lett 2019

オララツマブ (OLA) は軟部肉腫における初めての分子標的薬で，血小板由来成長因子受容体αを阻害し，細胞増殖を抑制する．標準薬ドキソルビシン（DOX）単剤と比較したphase-2臨床試験の結果で，OLAとDOXの併用療法が進行性軟部肉腫患者の予後を約2倍に延長したことで，2016年に欧米で迅速承認されたが，phase-3試験で，予後の改善を見出すことができず，2019年に承認が取り消された．しかし，OLAと他の化学療法との併用療法についての可能性は明らかにされていない．今回，標準治療レジメンであるAI (DOX + ifosfamide)療法とOLAとの併用療法の可能性について，患者由来組織同所移植モデルを用いて検討した．はじめに未分化多形肉腫患者の手術検体の一部をマウス皮下に移植した．増殖した腫瘍を分割してヌードマウスの大腿二頭筋内に移植し，マウスモデルを樹立した．このモデルをコントロール群，DOX群，OLA群，AI療法群， OLA+DOX群，OLA+AI群にランダムに振り分け，14日間の治療を行い，腫瘍サイズと体重を週2回測定した．治療後に腫瘍を切除し，Hematoxylin-Eosin (HE)染色とKi-67免疫組織化学染色で評価した．DOX群とOLA群を除く全ての群で, control群と比較し，有意に腫瘍の増殖が抑制されたが，AI群とOLA+DOX群の腫瘍増殖抑制効果は軽度であった．一方，OLA+AI群では腫瘍増殖が停止し，HE染色で広範な壊死を認め，Ki-67 陽性率は有意に減少した．全群で，有意な体重減少や大きな副作用はなかった．以上より，OLAとAI療法の併用療法は今後の臨床応用に期待できると考えた．

軟骨肉腫に対する非ステロイド性抗炎症薬ザルトプロフェンによるペルオキシゾーム増殖剤応答性受容体γとマトリックスメタロプロテアーゼ-2を介した抗腫瘍効果の解析

Cancer Med 2018

軟骨肉腫は2番目に多い原発性悪性骨腫瘍であるが，現在でも有効な化学療法や放射線治療がなく，手術以外の治療法がない．Peroxisome proliferator-activated receptor-γ（PPARγ）は脂肪細胞の分化に中心的な役割を果たす核内受容体型の転写因子で，近年，癌治療の新しい標的としても注目されている．我々は非ステロイド性鎮痛薬のザルトプロフェンが，PPARγを活性化することを初めて明らかにしました．本研究ではPPARγ活性化による治療の可能性が報告されている軟骨肉腫において，ザルトプロフェンのPPARγを標的とした抗腫瘍効果について解析した．まず，reporter gene assayを行い，ザルトプロフェンに強いPPARγ活性能を有することを確認し，western-blotやRT-PCRにより，ザルトプロフェンが軟骨肉腫細胞でPPARγの発現を濃度依存的に誘導することを見出した．続いて，proliferation assay，wound assay，invasion assay,　zymographyなどのin vitro解析の結果から，ザルトプロフェンが軟骨肉腫細胞の増殖能や遊走浸潤能，MMP-2の発現を濃度依存的に抑制することを明らかにし，これらの作用がPPARγを介していることを遺伝子ノックダウン実験で確認した．最後に，鎮痛のため，ザルトプロフェンを長期内服していた頸椎軟骨肉腫の1例において，内服前後の手術検体を組織学的に評価し，ザルトプロフェン内服後の検体でPPARγの増強とMMP-2の減弱を認めることを確認した．軟骨肉腫の多くは緩徐に成長するため，局所の浸潤や遠隔転移さえ抑制できれば，腫瘍と共存したままでも，長期生存が見込める可能性がある．そのため，本研究で明らかにした，ザルトプロフェンによるPPARγやMMP-2を介した遊走浸潤抑制効果が，手術困難となった軟骨肉腫患者の新しい治療戦略になると考えた。

患者由来腫瘍組織同所移植モデルを用いた肉腫の個別化医療に向けた取り組み

Cell Cycle 2017

骨軟部肉腫はいわゆる希少がんであり症例数の少なさゆえに新規治療開発や新規薬剤開発に困難を伴います．より臨床を反映した動物モデルを作成することで研究開発を促進することができないかと考え，肉腫患者の腫瘍組織をマウスの同所に移植する動物モデルを作成し，従来の皮下移植モデルと比較検討行いました．
まず成人発症の大腿横紋筋肉腫患者より腫瘍組織の提供を受けて，マウスの皮下に移植しました．腫瘍が生着することを確認した後に，筋肉内移植モデルとして大腿四頭筋と大腿二頭筋内に腫瘍を移植，皮下モデルも作成し，腫瘍の動態につき評価しました．
移植後早期から筋肉内移植モデルは皮下移植モデルと比較し有意に腫瘍は増大し，移植後10週の段階ですべての腫瘍は長径20mmに達しました．また，腫瘍の辺縁切除後のマウスを経時的に観察したところ，皮下移植モデルから再発は認めませんでしたが，筋肉内移植モデルでは高確率で再発しました．切除した腫瘍を病理学的に評価したところ，筋肉内モデルにおいてのみ周囲筋組織への浸潤が認められました．
横紋筋肉腫を用いた患者由来腫瘍組織同所移植モデルは腫瘍増殖速度が速く，辺縁切除で局所再発を来たし，病理学的に周囲への明らかな浸潤傾向を認めるため，皮下移植モデルと比較しより悪性度が高く，患者臨床を反映した動物モデルであるといえます．

ヨード担持量の減衰評価と抗菌力との関係

Journal of Microbial & Biochemical Technology 2017

液ヨード担持インプラント表面に担持されたポピドンヨードの経時的な減衰変化と、ヨード量と抗菌力の評価について検討した。
担持されたヨード量が生体内での減衰量を評価するため、動物モデルによる評価を実施した。ウサギの皮下組織内、膝関節内、大腿骨内にそれぞれヨード担持インプラントを埋植し、表面に残存するヨード担持量を経時的に評価を行った。結果、365日経過後も約30%のポピドンヨードが試験片表面に残存していることが示された。
担持されたヨード量と抗菌力との関係性を評価するため、基準値に対して80%、50%、20%、0%(=酸化被膜)にヨード担持量を調節した試験片を作成し、黄色ブドウ球菌 (ATCC 25923)と大腸菌 (MG 1655)を播種し、培養した後の細菌数を経時的に評価した。その結果、20%のヨード担持量においても有意な細菌数の減少を認めた。
以上より、ヨード担持インプラントの抗菌力が長期に持続する可能性が示唆された。

骨肉腫に対するGSK3βを標的として新規分子標的治療薬の研究

Oncotarget 2016

骨肉腫は小児の長管骨骨幹端に発生することの多い、骨原発悪性腫瘍である。治療方法は抗がん剤と手術であるが、抗がん剤の副作用が強い症例や抗腫瘍効果が小さい症例などが存在する。そこで、骨肉腫に対するGSK3βを標的とした新規分子標的治療法の研究を行った。4種の骨肉腫細胞株（HOS、143B、MG-63、SaOS-2）と1種の骨芽細胞株（hFOB1.19）を用いた。GSK3β阻害薬はAR-A014418、SB-216763を使用した。それぞれの細胞株におけるGSK3β活性について調べ、阻害薬投与による細胞増殖抑制効果とアポトーシスの評価を行った。また、骨肉腫細胞株における阻害薬投与後のβ-cateninの変化を調べ、骨肉腫に対するGSK3β阻害薬の作用経路を検討した。In vivoでは骨肉腫細胞同所移植マウスにGSK3β阻害薬を腹腔内投与し、その抗腫瘍効果とメカニズムを検証した。活性型GSK3βの発現は、全ての骨肉腫細胞株において亢進していた（下図）。GSK3β阻害薬により全ての骨肉腫細胞の増殖抑制効果が認められ、アポトーシスが誘導された。また、GSK3β特異的siRNAを用いたRNA干渉試験でも阻害薬と同様の効果を得た。GSK3β阻害薬によりβ-cateninのリン酸化と分解が抑制され、β-cateninの核内移行と転写補因子活性が誘導された。骨肉腫細胞同所移植マウスに阻害薬を投与した結果、投与後2週間目から腫瘍増殖抑制効果が確認され、摘出腫瘍においてβ-cateninの核集積が認められた。この結果、GSK3β阻害薬による治療効果にはWnt/β-catenin経路が関係していると考えられた。

脂肪系腫瘍由来幹細胞と正常脂肪由来幹細胞の分化能の違い

Clinical Orthopaedic and Related Research 2017

間葉系幹細胞（MSC）においてp53蛋白は骨芽細胞分化や脂肪細胞分化を抑制する。多くの悪性腫瘍においてp53機能は低下しているため、悪性腫瘍の中のMSCにおいてもp53機能が低下しているならば骨芽細胞分化や脂肪細胞分化への誘導療法が有効である可能性があり、この仮説を異型脂肪腫様腫瘍/高分化型脂肪肉腫（ALT/WDL）を用いて検証しようと考えた。なぜなら、ほとんどのALT/WDLにおいてp53機能低下につながるMDM2遺伝子の増幅が認められるからである。同一個体からALT/WDL組織と正常脂肪組織を採取し、MSCを分離培養し、骨芽細胞分化能と脂肪細胞分化能を比較する。ALT/WDL患者6人から、腫瘍切除術の際に3mlの正常脂肪組織とALT組織を採取した。脂肪分化能はオイルレッドO染色後、抽出液の吸光度を測定して定量的に評価した。骨分化能はALP染色およびアリザリンレッドS染色後に特定色域面積を測定し半定量的に評価した。ALPは骨芽細胞分化初期の代謝に関連し、アリザリンレッドはカルシウム沈着と関連する。次に我々は骨芽細胞分化の差異が生じたメカニズムを、MDM2特異的阻害薬Nutlin-3の濃度を変えて加えることで分析した。脂肪分化能においては、我々の予想に反して、正常脂肪由来MSCにおいてより多くの脂肪滴が認められた。ただし抽出液の吸光度測定結果（正常脂肪対ALT/WDL）(±SD)は0.34 (± 0.13) 対 0.25 (± 0.10; p =0.22)で、有意差はなかった。対照的に、骨芽細胞分化能においては、ALT/WDL由来MSCでより高い分化能を認めた。ALP染色に基づいた分化能の値（正常脂肪対ALT/WDL）(±SD)は1.00 対 17 (±35.8; p =0.04)であった。しかしながら、アリザリンレッドS染色に基づいた分化能の値（正常脂肪対ALT/WDL）(±SD)は1.00対4.24(±4.75; p = 0.58)で、有意差を認めなかった。ALP染色に基づく骨芽細胞分化能の差異はMDM2特異的阻害薬Nutlin-3濃度が上昇するにつれて減少した。ALT/WDL由来MSCにおけるMDM2遺伝子の増幅がより高い骨芽細胞分化能をもたらしている可能性が示唆された。

業績

英語論文（2010～）

2024年

Morinaga S, Han Q, Kubota Y, Mizuta K, Kang BM, Sato M, Bouvet M, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Higuchi T, Tsuchiya H, Hoffman RM.
Extensive Synergy Between Recombinant Methioninase and Eribulin Against Fibrosarcoma Cells But Not Normal Fibroblasts.
Anticancer Res. 2024 Mar;44(3):921-928.

Miwa S, Wu PK, Tsuchiya H.
Soft Tissue Sarcomas: Treatment and Management.
Cancers (Basel). 2024 Mar 4;16(5):1042.

Bangcoy MLS, Miwa S, Yamamoto N, Hayashi K, Nishida H, Takeuchi A, Igarashi K, Kato T, Taniguchi Y, Morinaga S, Asano Y, Tsuchiya H.
Tumor-induced Osteomalacia Successfully Treated by En Bloc Tumor Excision and Reconstruction Using a Tumor-bearing Frozen Autograft: A Case Report.
Anticancer Res. 2024 Apr;44(4):1791-1797.

Higuchi T, Taninaka A, Yoshimizu R, Hayashi K, Miwa S, Yamamoto N, Tsuchiya H, Demura S.
Intraoperative Fracture during the Insertion of Advanced Locking Screws (T2 Alpha Femur Retrograde Intramedullary Nailing System): Report of Two Cases and Identifying Causes and Prevention.
J Clin Med. 2024 Apr 19;13(8):2393.

2023年

Araki Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Abe K, Taniguchi Y, Yonezawa H, Morinaga S, Asano Y, Nojima T, Taki J, Tsuchiya H.
A Viability Analysis of Tumor-Bearing Frozen Autograft for the Reconstruction After Resection of Malignant Bone Tumors Using 99m Tc-MDP Scintigraphy.
Clin Nucl Med. 2023 Jan 1;48(1):25-34.

Higuchi T, Takeuchi A, Munesue S, Yamamoto N, Hayashi K, Harashima A, Yamamoto Y, Tsuchiya H.
A nonsteroidal anti-inflammatory drug, zaltoprofen, inhibits the growth of extraskeletal chondrosarcoma cells by inducing PPARγ, p21, p27, and p53.
Cell Cycle. 2023 Apr;22(8):939-950.

Yamamuro Y, Kabata T, Nojima T, Hayashi K, Tokoro M, Kajino Y, Inoue D, Ohmori T, Yoshitani J, Ueno T, Ueoka K, Taninaka A, Kataoka T, Saiki Y, Yanagi Y, Tsuchiya H.
Combined adipose-derived mesenchymal stem cell and antibiotic therapy can effectively treat periprosthetic joint infection in rats.
Sci Rep. 2023 Mar 9;13(1):3949.

Miwa S, Yamamoto N, Tsuchiya H. Sarcoma: Molecular Pathology, Diagnostics, and Therapeutics.
Int J Mol Sci. 2023 Mar 19;24(6):5833.

Morinaga S, Yamamoto N, Tokoro M, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Taniguchi Y, Asano Y, Nojima T, Tsuchiya H.
Antibacterial effect and biological reaction of calcium phosphate cement impregnated with iodine for use in bone defects.
J Biomater Appl. 2023 May;37(10):1716-1723.

Asano Y, Yamamoto N, Demura S, Hayashi K, Takeuchi A, Kato S, Miwa S, Igarashi K, Higuchi T, Taniguchi Y, Morinaga S, Sone T, Okuda M, Matsumoto I, Yano S, Tsuchiya H.
Novel predictors of immune checkpoint inhibitor response and prognosis in advanced non-small-cell lung cancer with bone metastasis.
Cancer Med. 2023 Jun;12(11):12425-12437.

Bangcoy MLS, Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Taniguchi Y, Morinaga S, Asano Y, Tsuchiya H.
Clinical Benefits of LigaSure™ for Surgical Amputation Without Tourniquet Use in Patients With Musculoskeletal Tumor.
Anticancer Res. 2023 May;43(5):2127-2133.

Morinaga S, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Taniguchi Y, Asano Y, Nojima T, Tsuchiya H.
Kimura’s Disease Diagnosed in the Department of Orthopedic Surgery Treated With Wide Excision: Report of Two Cases.
In Vivo. 2023 May-Jun;37(3):1373-1378.

Araki Y, Asano N, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Abe K, Taniguchi Y, Yonezawa H, Morinaga S, Asano Y, Yoshida T, Hanayama R, Matsuzaki J, Ochiya T, Kawai A, Tsuchiya H.
A validation study for the utility of serum microRNA as a diagnostic and prognostic marker in patients with osteosarcoma.
Oncol Lett. 2023 Apr 13;25(6):222.

Aiba H, Kamei M, Ito Y, Takeda R, Yamada S, Okamoto H, Hayashi K, Miwa S, Kawaguchi Y, Saito S, Sakai T, Murakami H, Kimura H. Outcomes of Window Therapy with Carboplatin and Ifosfamide for Pediatric Osteosarcoma: A Case Series Children (Basel). 2023 Apr 17;10(4):736.

Taninaka A, Kabata T, Hayashi K, Kajino Y, Inoue D, Ohmori T, Ueoka K, Yamamuro Y, Kataoka T, Saiki Y, Yanagi Y, Ima M, Iyobe T, Tsuchiya H.
Chondroprotective Effects of Chondrogenic Differentiated Adipose-Derived Mesenchymal Stem Cells Sheet on Degenerated Articular Cartilage in an Experimental Rabbit Model.
Bioengineering (Basel). 2023 May 10;10(5):574.

Kawai M, Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Taniguchi Y, Araki Y, Yonezawa H, Nojima T, Tsuchiya H.
Needle tract seeding of a sclerosing epithelioid fibrosarcoma in a biopsy tract: a case report.
BMC Musculoskelet Disord. 2023 Jun 3;24(1):454.

Takimoto R, Kamigaki T, Ito H, Saito M, Takizawa K, Soejima K, Yasuda H, Ohgino K, Terai H, Tomita K, Miura M, Mizukoshi E, Miyashita T, Nakamoto Y, Hayashi K, Miwa S, Kitahara M, Takeuchi A, Kimura H, Mochizuki T, Sugie H, Seino KI, Yamada T, Takeuchi S, Makita K, Naitoh K, Yasumoto K, Yoshida Y, Inoue H, Kotake K, Ohshima K, Noda SE, Okamoto M, Yoshimoto Y, Okada S, Ibe H, Oguma E, Goto S; CITEG.
Safety evaluation of immune-cell therapy for malignant tumor in the Cancer Immune-cell Therapy Evaluation Group (CITEG).
Cytotherapy. 2023 Nov;25(11):1229-1235.

Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Bangcoy MLS, Taniguchi Y, Morinaga S, Asano Y, Tsuchiya H.
Graft Survivals after Reconstruction Using Tumor-Bearing Frozen Bone in the Extremities.
Cancers (Basel). 2023 Aug 2;15(15):3926.

Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Taniguchi Y, Morinaga S, Asano Y, Nojima T, Tsuchiya H.
Case Report: Unresectable pulmonary metastases of a giant cell tumor of bone treated with denosumab: a case report and review of literature.
Front Oncol. 2023 Aug 16;13:1230074.

Miwa S, Tanaka T, Aiba H, Yamada S, Otsuka T, Tsuchiya H.
Multiple Bone Cysts Caused by Hyperparathyroidism: A Case Report and Review of the Literature.
Cancer Diagn Progn. 2023 Sep 3;3(5):590-596.

Aiba H, Miwa S, Murakami H, Kimura H.
Special Issue: “Pediatric Orthopedic Malignancy: Types, Symptoms, and Treatment”
Children (Basel). 2023 Sep 13;10(9):1545.

Higuchi T, Yamamoto N, Hayashi K, Miwa S, Igarashi K, Tsuchiya H, Hoffman RM.
High Clinical Concordance of Drug Resistance in Patient-derived Orthotopic Xenograft (PDOX) Mouse Models: First Step to Validated Precise Individualized Cancer Chemotherapy.
Anticancer Res. 2023 Oct;43(10):4277-4284.

2022年

Asano Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Yonezawa H, Araki Y, Morinaga S, Saito S, Nojima T, Tsuchiya H.
Impact of Tumor Growth Speed of Primary Lesions on the Clinical Outcomes of Appendicular Skeletal Metastases.
Anticancer Res. 2022 Jan;42(1):229-236.

Asano Y, Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Yonezawa H, Araki Y, Morinaga S, Nojima T, Ikeda H, Tsuchiya H.
A scoring system combining clinical, radiological, and histopathological examinations for differential diagnosis between lipoma and atypical lipomatous tumor/well-differentiated liposarcoma.
Sci Rep. 2022 Jan 7;12(1):237.

Araki Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Abe K, Taniguchi Y, Yonezawa H, Morinaga S, Asano Y, Tsuchiya H.
Pretreatment Neutrophil Count and Platelet-lymphocyte Ratio as Predictors of Metastasis in Patients With Osteosarcoma.
Anticancer Res. 2022 Feb;42(2):1081-1089.

Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Tsuchiya H.
Therapeutic Targets and Emerging Treatments in Advanced Chondrosarcoma.
Int J Mol Sci. 2022 Jan 20;23(3):1096.

Takeuchi A, Yamamoto N, Ohmori T, Hayashi K, Miwa S, Igarashi K, Higuchi T, Abe K, Yonezawa H, Morinaga S, Araki Y, Asano Y, Saito S, Tsuchiya H.
Primary total knee arthroplasty assisted by computed tomography-free navigation for secondary knee osteoarthritis following massive calcium phosphate cement packing for distal femoral giant-cell bone tumor treatment: a case report.
BMC Musculoskelet Disord. 2022 Feb 22;23(1):170.

Araki Y, Yamamoto N, Maeda T, Kimura H, Ota T, Shimozaki S, Kato T, Inoue D, Higuchi T, Abe K, Taniguchi Y, Tsuchiya H.
Management of Soft-tissue Tumors With a Size of 2-5 cm, Including Malignancy.
Anticancer Res. 2022 Mar;42(3):1555-1562.

Hayashi K, Tsuchiya H.
The role of surgery in the treatment of metastatic bone tumor.
Int J Clin Oncol. 2022 Aug;27(8):1238-1246.

Kawai A, Araki N, Ae K, Akiyama T, Ozaki T, Kawano H, Kunisada T, Sumi M, Takahashi S, Tanaka K, Tsukushi S, Naka N, Nishida Y, Miyachi M, Yamamoto N, Yoshida A, Yonemoto T, Yoshida M, Iwata S.
Japanese Orthopaedic Association (JOA) clinical practice guidelines on the management of soft tissue tumors 2020 – Secondary publication.
J Orthop Sci. 2022 May;27(3):533-550.

Yamamoto N, Araki Y, Tsuchiya H.
Joint-preservation surgery for bone sarcoma in adolescents and young adults.
Int J Clin Oncol. 2023 Jan;28(1):12-27.

Azurin ES, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Yonezawa H, Morinaga S, Asano Y, Saito S, Tsuchiya H.
Monostotic Fibrous Dysplasia in the Femur Strongly Expressing RANKL With Concomitant Osteoporotic Vertebral Compression Fracture: A Case Report.
Cancer Diagn Progn. 2022 Jan 3;2(1):93-100.

Asano Y, Yamamoto N, Demura S, Hayashi K, Takeuchi A, Kato S, Miwa S, Igarashi K, Higuchi T, Yonezawa H, Araki Y, Morinaga S, Saito S, Sone T, Kasahara K, Tsuchiya H.
The Therapeutic Effect and Clinical Outcome of Immune Checkpoint Inhibitors on Bone Metastasis in Advanced Non-Small-Cell Lung Cancer.
Front Oncol. 2022 Apr 1;12:871675.

Higuchi T, Yamamoto N, Nishida H, Hayashi K, Takeuchi A, Tsuchiya H.
Treatment of infected calcific myonecrosis with chronically discharging sinus caused by iatrogenic aspiration: A case report.
Int J Surg Case Rep. 2022 Jun;95:107145.

Okamoto H, Kawaguchi Y, Miwa S, Aiba H, Senda H, Murakami S, Hayakawa K, Joyo Y, Murakami H, Kimura H.
Reverse lateral upper arm flaps for treating large soft tissue defects extending from the elbow to the forearm.
Case Reports Plast Surg Hand Surg. 2022 May 19;9(1):145-150.

Morinaga S, Takeuchi A, Yamamoto N, Hayashi K, Miwa S, Igarashi K, Yonezawa H, Asano Y, Saito S, Nojima T, Tsuchiya H.
Compartment-specific Clinical Outcomes in Patients With Soft Tissue Sarcomas of the Thigh.
Anticancer Res. 2022 Jun;42(6):3143-3150.

Yonezawa H, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Morinaga S, Asano Y, Saito S, Tome Y, Ikeda H, Nojima T, Tsuchiya H.
Dedifferentiated chondrosarcoma of the middle finger arising from a solitary enchondroma: A case report.
World J Clin Cases. 2022 Apr 6;10(10):3297-3305.

Takemori T, Kawamoto T, Hara H, Fukase N, Fujiwara S, Fujita I, Fujimoto T, Morishita M, Kitayama K, Yahiro S, Miyamoto T, Saito M, Sugaya J, Hayashi K, Kawashima H, Torigoe T, Nakamura T, Kondo H, Wakamatsu T, Watanuki M, Kito M, Tsukushi S, Nagano A, Outani H, Toki S, Nishimura S, Kobayashi H, Watanabe I, Demizu Y, Sasaki R, Fukumoto T, Niikura T, Kuroda R, Akisue T.
Clinical Outcome of Patients with Pelvic and Retroperitoneal Bone and Soft Tissue Sarcoma: A Retrospective Multicenter Study in Japan.
Cancers (Basel). 2022 Jun 20;14(12):3023.

Yonezawa H, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Morinaga S, Asano Y, Saito S, Tada K, Nojima T, Tsuchiya H.
Do Liquid Nitrogen-treated Tumor-bearing Nerve Grafts Have the Capacity to Regenerate, and Do They Pose a Risk of Local Recurrence? A Study in Rats.
Clin Orthop Relat Res. 2022 Dec 1;480(12):2442-2455.

Higuchi T, Igarashi K, Yamamoto N, Hayashi K, Kimura H, Miwa S, Bouvet M, Tsuchiya H, Hoffman RM.
Review: Precise sarcoma patient-derived orthotopic xenograft (PDOX) mouse models enable identification of novel effective combination therapies with the cyclin-dependent kinase inhibitor palbociclib: A strategy for clinical application.
Front Oncol. 2022 Aug 8;12:957844.

Annen R, Kato S, Demura S, Miwa S, Yokka A, Shinmura K, Yokogawa N, Yonezawa N, Kobayashi M, Kurokawa Y, Gabata T, Tsuchiya H.
Tumor-Specific Immunoenhancing Effects after Local Cryoablation for Metastatic Bone Tumor in a Mouse Model.
Int J Mol Sci. 2022 Aug 21;23(16):9445.

Asano Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Taniguchi Y, Morinaga S, Tada K, Nojima T, Tsuchiya H.
Clinical Outcomes of Limb-sparing Tumor Surgery With Vascular Reconstruction for Bone and Soft-tissue Tumors.
Anticancer Res. 2022 Sep;42(9):4619-4626.

Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Tsuchiya H.
Surgical Site Infection after Bone Tumor Surgery: Risk Factors and New Preventive Techniques.
Cancers (Basel). 2022 Sep 19;14(18):4527.

Takeuchi A, Endo M, Kawai A, Nishida Y, Terauchi R, Matsumine A, Aiba H, Nakamura T, Tandai S, Ozaki T, Hoshi M, Kayano D, Okuda M, Yamamoto N, Hayashi K, Miwa S, Igarashi K, Yoshimura K, Nomura A, Murayama T, Tsuchiya H.
Randomized placebo-controlled double-blind phase II study of zaltoprofen for patients with diffuse-type and unresectable localized tenosynovial giant cell tumors: The REALIZE study.
Front Oncol. 2022 Sep 21;12:900010.

Araki Y, Yamamoto N, Tanzawa Y, Higashi T, Kuchiba A, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Endo M, Kobayashi E, Tsuchiya H, Kawai A.
Family cancer history and smoking habit associated with sarcoma in a Japanese population study.
Sci Rep. 2022 Oct 12;12(1):17129.

Asano Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Taniguchi Y, Morinaga S, Horimoto T, Nakai M, Kadono Y, Nojima T, Tsuchiya H.
Case report: Complete remission of bone metastasis from renal cell carcinoma in histopathological examination after treatment with immune checkpoint inhibitors.
Front Immunol. 2022 Sep 27;13:980456.

Araki Y, Yamamoto N, Higuchi T, Tsuchiya H.
A Traumatic Intermuscular Hematoma Mimicking a Soft-tissue Tumor: A Case Report and Literature Review.
In Vivo. 2022 Nov-Dec;36(6):3018-3022.

Araki Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Abe K, Taniguchi Y, Yonezawa H, Morinaga S, Asano Y, Nojima T, Tsuchiya H.
Primary Osteosarcoma in Patients Over 75 Years of Age: A Case Series and Literature Review.
In Vivo. 2022 Nov-Dec;36(6):2899-2909.

Morinaga S, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Yonezawa H, Asano Y, Saito S, Tsuchiya H.
Insufficiency fracture of the supra-acetabulum that required differentiation from a pathological fracture secondary to a malignant bone tumor: a case report.
J Med Case Rep. 2022 Nov 16;16(1):436.

Azurin ES, Yamamoto N, Hayashi K, Takeuchi A, Tada K, Miwa S, Igarashi K, Higuchi T, Yonezawa H, Morinaga S, Asano Y, Saito S, Tsuchiya H.
Clinical Outcomes of Soft Tissue Sarcoma around the Elbow Joint: A Retrospective Single Institution Study.
Sarcoma. 2022 Dec 17;2022:1087726.

2021年

Higuchi T, Igarashi K, Yamamoto N, Hayashi K, Kimura H, Miwa S, Bouvet M, Tsuchiya H, Hoffman RM.
Multikinase-Inhibitor Screening in Drug-resistant Osteosarcoma Patient-derived Orthotopic Xenograft Mouse Models Identifies the Clinical Potential of Regorafenib.
Cancer Genomics Proteomics. 2021 Sep-Oct;18(5):637-643.

Araki Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Abe K, Taniguchi Y, Yonezawa H, Morinaga S, Asano Y, Tsuchiya H.
Clinical outcomes of frozen autograft reconstruction for the treatment of primary bone sarcoma in adolescents and young adults.
Sci Rep. 2021 Aug 27;11(1):17291.

Xu G, Aiba H, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Araki Y, Saito S, Yoshimura K, Murakami H, Tsuchiya H, Kawai A.
Efficacy of perioperative chemotherapy for synovial sarcoma: a retrospective analysis of a Nationwide database in Japan.
BMC Cancer. 2021 Jul 3;21(1):773.

Hayashi K, Hiraoka K, Akiyama T, Iwata S, Tsuchiya H, Kawai A.
Benefit of surgical resection of distant metastasis in soft tissue sarcoma: a systematic review.
Jpn J Clin Oncol. 2021 Jul 1;51(7):1088-1093.

Araki Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Abe K, Taniguchi Y, Yonezawa H, Morinaga S, Asano Y, Tsuchiya H.
Delayed Initiation of Treatment Is Associated With Metastasis of Malignant Bone Tumor.
Anticancer Res. 2021 Jun;41(6):2993-2999.

Yonezawa H, Hayashi K, Yamamoto N, Takeuchi A, Tada K, Miwa S, Igarashi K, Kimura H, Aoki Y, Morinaga S, Araki Y, Asano Y, Sakurakichi K, Ikeda H, Nojima T, Tsuchiya H.
Significant Improvement After Surgery for a Symptomatic Osteoblastoma in a Patient with Camurati-Engelmann Disease: Case Report and Literature Review.
Calcif Tissue Int. 2021 Jun;108(6):819-824.

Araki Y, Aiba H, Yoshida T, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Nguyen TD, Ishii KA, Nojima T, Takahashi S, Murakami H, Tsuchiya H, Hanayama R.
Osteosarcoma-Derived Small Extracellular Vesicles Enhance Tumor Metastasis and Suppress Osteoclastogenesis by miR-146a-5p.
Front Oncol. 2021 May 4;11:667109.

Miwa S, Yamamoto N, Tsuchiya H.
Bone and Soft Tissue Tumors: New Treatment Approaches.
Cancers (Basel). 2021 Apr 12;13(8):1832.

Higuchi T, Han Q, Sugisawa N, Yamamoto J, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
Combination Methionine-methylation-axis Blockade: A Novel Approach to Target the Methionine Addiction of Cancer.
Cancer Genomics Proteomics. 2021 Mar-Apr;18(2):113-120.

Tsuchiya H.
Heritage and Invention – Dream, Dare and Do.
J Orthop Sci. 2021 Mar;26(2):201-202.

Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Tada K, Higuchi T, Yonezawa H, Morinaga S, Araki Y, Asano Y, Ikeda H, Nojima T, Tsuchiya H.
Diagnostic accuracies of intraoperative frozen section and permanent section examinations for histological grades during open biopsy of bone tumors.
Int J Clin Oncol. 2021 Mar;26(3):613-619.

Asano Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Yonezawa H, Araki Y, Morinaga S, Kasahara K, Sone T, Tsuchiya H.
Complete Response of Bone Metastasis in Non-small Cell Lung Cancer With Pembrolizumab: Two Case Reports.
Anticancer Res. 2021 Mar;41(3):1693-1699.

Hayashi K, Yamamoto N, Takeuchi A, Miwa S, Igarashi K, Araki Y, Yonezawa H, Morinaga S, Asano Y, Tsuchiya H.
Long-term survival in a patient with Hutchinson-Gilford progeria syndrome and osteosarcoma: A case report.
World J Clin Cases. 2021 Feb 6;9(4):854-863.

Araki Y, Hayashi K, Yamamoto N, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Abe K, Taniguchi Y, Yonezawa H, Morinaga S, Asano Y, Nojima T, Tsuchiya H.
Reconstruction using a frozen autograft for a skull and humeral lesion of synchronous multicentric osteosarcoma after undergoing successful neoadjuvant chemotherapy: a case report and review of the literature.
BMC Surg. 2021 Jan 22;21(1):56.

2020年

Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Higuchi T, Yamamoto N, Hayashi K, Kimura H, Miwa S, Singh SR, Tsuchiya H, Hoffman RM.
Eribulin Regresses a Doxorubicin-resistant Dedifferentiated Liposarcoma in a Patient-derived Orthotopic Xenograft Mouse Model.
Cancer Genomics Proteomics. 2020 Jul-Aug;17(4):351-358.

Langit MB, Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Tada K, Higuchi T, Yonezawa H, Morinaga S, Araki Y, Asano Y, Tsuchiya H.
Risk Factors for Postoperative Deep Infection After Malignant Bone Tumor Surgery of the Extremities.
Anticancer Res. 2020 Jun;40(6):3551-3557.

Igarashi K, Kawaguchi K, Zhao M, Han Q, Tan Y, Kiyuna T, Miyake K, Higuchi T, Nelson SD, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Singh SR, Tsuchiya H, Hoffman RM.
Recombinant Methioninase Combined With Tumor-targeting Salmonella typhimurium A1-R Induced Regression in a PDOX Mouse Model of Doxorubicin-resistant Dedifferentiated Liposarcoma.
Anticancer Res. 2020 May;40(5):2515-2523.

Igarashi K, Kawaguchi K, Yamamoto N, Hayashi K, Kimura H, Miwa S, Higuchi T, Taniguchi Y, Yonezawa H, Araki Y, Morinaga S, Misra S, Nelson SD, Dry SM, Li Y, Odani A, Singh SR, Tsuchiya H, Hoffman RM.
A Novel Anionic-phosphate-platinum Complex Effectively Targets a Cisplatinum-resistant Osteosarcoma in a Patient-derived Orthotopic Xenograft Mouse Model.
Cancer Genomics Proteomics. 2020 May-Jun;17(3):217-223.

Araki Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Taniguchi Y, Yonezawa H, Morinaga S, Nojima T, Tsuchiya H.
Cystic extraskeletal osteosarcoma: Three case reports and review of the literature.
Mol Clin Oncol. 2020 May;12(5):468-474.

Higuchi T, Sugisawa N, Yamamoto J, Oshiro H, Han Q, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Tan Y, Kuchipudi S, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
The combination of oral-recombinant methioninase and azacitidine arrests a chemotherapy-resistant osteosarcoma patient-derived orthotopic xenograft mouse model.
Cancer Chemother Pharmacol. 2020 Feb;85(2):285-291.

Higuchi T, Han Q, Miyake K, Oshiro H, Sugisawa N, Tan Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
Combination of oral recombinant methioninase and decitabine arrests a chemotherapy-resistant undifferentiated soft-tissue sarcoma patient-derived orthotopic xenograft mouse model.
Biochem Biophys Res Commun. 2020 Feb 26;523(1):135-139.

Xu G, Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Higuchi T, Taniguchi Y, Araki Y, Yonezawa H, Morinaga S, Tsuchiya H.
Pedicle frozen autograft-prosthesis composite reconstructions for malignant bone tumors of the proximal femur.
BMC Musculoskelet Disord. 2020 Feb 6;21(1):81.

Xu G, Yamamoto N, Nojima T, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Tsuchiya H.
The process of bone regeneration from devitalization to revitalization after pedicle freezing with immunohistochemical and histological examination in rabbits.
Cryobiology. 2020 Feb 1;92:130-137.

Abe K, Yamamoto N, Domoto T, Bolidong D, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Inatani H, Aoki Y, Higuchi T, Taniguchi Y, Yonezawa H, Araki Y, Aiba H, Minamoto T, Tsuchiya H.
Glycogen synthase kinase 3β as a potential therapeutic target in synovial sarcoma and fibrosarcoma.
Cancer Sci. 2020 Feb;111(2):429-440.

Igarashi K, Kawaguchi K, Zhao M, Kiyuna T, Miyake K, Miyake M, Nelson SD, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Higuchi T, Singh SR, Tsuchiya H, Hoffman RM.
Exquisite Tumor Targeting by Salmonella A1-R in Combination with Caffeine and Valproic Acid Regresses an Adult Pleomorphic Rhabdomyosarcoma Patient-Derived Orthotopic Xenograft Mouse Model.
Transl Oncol. 2020 Feb;13(2):393-400.

Xu G, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Taniguchi Y, Araki Y, Yonezawa H, Morinaga S, Tsuchiya H.
The accuracy of different FRAX tools in predicting fracture risk in Japan: A comparison study.
J Orthop Surg (Hong Kong). 2020 Jan-Apr;28(2):2309499020917276.

Higuchi T, Yamamoto J, Sugisawa N, Tashiro Y, Nishino H, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
PPARγ Agonist Pioglitazone in Combination With Cisplatinum Arrests a Chemotherapy-resistant Osteosarcoma PDOX Model.
Cancer Genomics Proteomics. 2020 Jan-Feb;17(1):35-40.

Igarashi K, Kawaguchi K, Murakami T, Miyake K, Kiyuna T, Miyake M, Hiroshima Y, Higuchi T, Oshiro H, Nelson SD, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Singh SR, Tsuchiya H, Hoffman RM.
Patient-derived orthotopic xenograft models of sarcoma.
Cancer Lett. 2020 Jan 28;469:332-339.

2019年

Takeuchi A, Yamamoto N, Hayashi K, Matsubara H, Miwa S, Igarashi K, Tsuchiya H.
Joint-preservation surgery for pediatric osteosarcoma of the knee joint.
Cancer Metastasis Rev. 2019 Dec;38(4):709-722.

Miwa S, Okamoto H, Yamada S, Kawaguchi Y, Endo K, Aiba H, Hayashi K, Kimura H, Sekiya I, Otsuka T, Tsuchiya H.
Distribution of Solitary and Multiple Enchondromas of the Hand.
In Vivo. 2019 Nov-Dec;33(6):2235-2240.

Taniguchi Y, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Igarashi K, Higuchi T, Abe K, Yonezawa H, Araki Y, Morinaga S, Kamei J, Nugroho AE, Kaneda T, Morita H, Tsuchiya H.
Anti-tumor Effects of Cyclolinopeptide on Giant-cell Tumor of the Bone.
Anticancer Res. 2019 Nov;39(11):6145-6153.

Shirai T, Tsuchiya H, Terauchi R, Tsuchida S, Mizoshiri N, Mori Y, Takeuchi A, Hayashi K, Yamamoto N, Ikoma K, Kubo T.
A retrospective study of antibacterial iodine-coated implants for postoperative infection.
Medicine (Baltimore). 2019 Nov;98(45):e17932.

Higuchi T, Sugisawa N, Miyake K, Oshiro H, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Kline Z, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
Pioglitazone, an agonist of PPARγ, reverses doxorubicin-resistance in an osteosarcoma patient-derived orthotopic xenograft model by downregulating P-glycoprotein expression.
Biomed Pharmacother. 2019 Oct;118:109356.

Yamamoto N, Tsuchiya H.
Treatment of chordoma-where is it going?
J Spine Surg. 2019 Sep;5(3):387-389.

Higuchi T, Sugisawa N, Miyake K, Oshiro H, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Kline Z, Belt P, Chawla SP, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
Combination Treatment With Sorafenib and Everolimus Regresses a Doxorubicin-resistant Osteosarcoma in a PDOX Mouse Model.
Anticancer Res. 2019 Sep;39(9):4781-4786.

Higuchi T, Oshiro H, Miyake K, Sugisawa N, Han Q, Tan Y, Park J, Zhang Z, Razmjooei S, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Bouvet M, Chawla SP, Singh SR, Tsuchiya H, Hoffman RM.
Oral Recombinant Methioninase, Combined With Oral Caffeine and Injected Cisplatinum, Overcome Cisplatinum-Resistance and Regresses Patient-derived Orthotopic Xenograft Model of Osteosarcoma.
Anticancer Res. 2019 Sep;39(9):4653-4657.

Higuchi T, Sugisawa N, Miyake K, Oshiro H, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
The Combination of Olaratumab with Doxorubicin and Cisplatinum Regresses a Chemotherapy-Resistant Osteosarcoma in a Patient-Derived Orthotopic Xenograft Mouse Model.
Transl Oncol. 2019 Sep;12(9):1257-1263.

Zekry KM, Yamamoto N, Hayashi K, Takeuchi A, Araki Y, Alkhooly AZA, Abd-Elfattah AS, Fouly EH, Elsaid ANS, Tsuchiya H.
Surgical treatment of chondroblastoma using extended intralesional curettage with phenol as a local adjuvant.
J Orthop Surg (Hong Kong). 2019 Sep-Dec;27(3):2309499019861031.

Higuchi T, Sugisawa N, Miyake K, Oshiro H, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Chawla SP, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
Sorafenib and Palbociclib Combination Regresses a Cisplatinum-resistant Osteosarcoma in a PDOX Mouse Model.
Anticancer Res. 2019 Aug;39(8):4079-4084.

Abe K, Yamamoto N, Hayashi K, Takeuchi A, Tsuchiya H.
Caffeine citrate enhanced cisplatin antitumor effects in osteosarcoma and fibrosarcoma in vitro and in vivo.
BMC Cancer. 2019 Jul 15;19(1):689.

Higuchi T, Yamamoto N, Hayashi K, Takeuchi A, Abe K, Taniguchi Y, Araki Y, Tsuchiya H.
C-arm cone-beam computed tomography-guided minimally invasive open excision of an osteoid osteoma undetectable on fluoroscopy: A case report.
Int J Surg Case Rep. 2019;61:14-19.

Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Miyake M, Nelson SD, Russell TA, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Higuchi T, Singh SR, Tsuchiya H, Hoffman RM.
Pazopanib regresses a doxorubicin-resistant synovial sarcoma in a patient-derived orthotopic xenograft mouse model.
Tissue Cell. 2019 Jun;58:107-111.

Higuchi T, Miyake K, Sugisawa N, Oshiro H, Zhang Z, Razmjooei S, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
The combination of olaratumab with gemcitabine and docetaxel arrests a chemotherapy-resistant undifferentiated soft-tissue sarcoma in a patient-derived orthotopic xenograft mouse model.
Cancer Chemother Pharmacol. 2019 Jun;83(6):1075-1082.

Higuchi T, Miyake K, Sugisawa N, Oshiro H, Zhang Z, Razmjooei S, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
Olaratumab combined with doxorubicin and ifosfamide overcomes individual doxorubicin and olaratumab resistance of an undifferentiated soft-tissue sarcoma in a PDOX mouse model.
Cancer Lett. 2019 Jun 1;451:122-127.

Higuchi T, Miyake K, Oshiro H, Sugisawa N, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Chawla SP, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
Trabectedin and irinotecan combination regresses a cisplatinum-resistant osteosarcoma in a patient-derived orthotopic xenograft nude-mouse model.
Biochem Biophys Res Commun. 2019 May 28;513(2):326-331.

Yamamoto N, Hayashi K, Tsuchiya H.
Progress in biological reconstruction and enhanced bone revitalization for bone defects.
J Orthop Sci. 2019 May;24(3):387-392.

Takeuchi A, Matsubara H, Yamamoto N, Hayashi K, Miwa S, Igarashi K, Inatani H, Tsuchiya H.
Successful treatment of pathologic femoral shaft fracture associated with large arteriovenous malformations using a 3-dimensional external fixator and teriparatide: a case report.
BMC Surg. 2019 Apr 5;19(1):35.

Abe K, Yamamoto N, Hayashi K, Takeuchi A, Kato S, Miwa S, Igarashi K, Inatani H, Aoki YU, Higuchi T, Taniguchi Y, Tsuchiya H.
Determining Patient Satisfaction and Treatment Desires in Patients With Musculoskeletal Sarcoma of the Knee After Joint-preservation Surgery Using a Questionnaire Survey.
Anticancer Res. 2019 Apr;39(4):1965-1969.

Abe K, Yamamoto N, Hayashi K, Takeuchi A, Kato S, Miwa S, Igarashi K, Inatani H, Aoki YU, Higuchi T, Taniguchi Y, Tsuchiya H.
Satisfaction After Joint-preservation Surgery in Patients With Musculoskeletal Knee Sarcoma Based on Various Scores.
Anticancer Res. 2019 Apr;39(4):1959-1964.

Higuchi T, Oshiro H, Zhang Z, Miyake K, Sugisawa N, Katsuya Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Igarashi K, Zhao M, Bouvet M, Singh SR, Tsuchiya H, Hoffman RM.
Osimertinib Regresses an EGFR-Mutant Cisplatinum- Resistant Lung Adenocarcinoma Growing in the Brain in Nude Mice.
Transl Oncol. 2019 Apr;12(4):640-645.

Higuchi T, Kawaguchi K, Miyake K, Oshiro H, Zhang Z, Razmjooei S, Wangsiricharoen S, Igarashi K, Yamamoto N, Hayashi K, Kimura H, Miwa S, Nelson SD, Dry SM, Li Y, Chawla SP, Eilber FC, Singh SR, Tsuchiya H, Hoffman RM.
The combination of gemcitabine and nab-paclitaxel as a novel effective treatment strategy for undifferentiated soft-tissue sarcoma in a patient-derived orthotopic xenograft (PDOX) nude-mouse model.
Biomed Pharmacother. 2019 Mar;111:835-840.

Takeuchi A, Nomura A, Yamamoto N, Hayashi K, Igarashi K, Tandai S, Kawai A, Matsumine A, Miwa S, Nishida Y, Nakamura T, Terauchi R, Hoshi M, Kunisada T, Endo M, Yoshimura K, Murayama T, Tsuchiya H.
Randomized placebo-controlled double-blind phase II study of zaltoprofen for patients with diffuse-type and unresectable localized tenosynovial giant cell tumors: a study protocol.
BMC Musculoskelet Disord. 2019 Feb 9;20(1):68.

Miwa S, Shirai T, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Tsuchiya H.
Current and Emerging Targets in Immunotherapy for Osteosarcoma.
J Oncol. 2019 Jan 1;2019:7035045.

Miwa S, Shirai T, Yamamoto N, Hayashi K, Takeuchi A, Tada K, Kajino Y, Higuchi T, Abe K, Aiba H, Taniguchi Y, Tsuchiya H.
Risk factors for surgical site infection after malignant bone tumor resection and reconstruction.
BMC Cancer. 2019 Jan 8;19(1):33.

Zekry KM, Yamamoto N, Hayashi K, Takeuchi A, Alkhooly AZA, Abd-Elfattah AS, Elsaid ANS, Ahmed AR, Tsuchiya H.
Reconstruction of intercalary bone defect after resection of malignant bone tumor.
J Orthop Surg (Hong Kong). 2019 Jan-Apr;27(1):2309499019832970.

Miwa S, Yamamoto N, Hayashi K, Takeuchi A, Igarashi K, Tsuchiya H.
Therapeutic Targets for Bone and Soft-Tissue Sarcomas.
Int J Mol Sci. 2019 Jan 4;20(1):170.

Tome Y, Kiyuna T, Uehara F, Bouvet M, Tsuchiya H, Kanaya F, Hoffman RM.
Imaging the interaction of αv integrin-GFP in osteosarcoma cells with RFP-expressing host stromal cells and tumor-scaffold collagen in the primary and metastatic tumor microenvironment.
J Cell Biochem. 2019 Jan;120(1):283-289.

2018年

Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Miyake M, Li Y, Nelson SD, Dry SM, Singh AS, Elliott IA, Russell TA, Eckardt MA, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM.
Temozolomide regresses a doxorubicin-resistant undifferentiated spindle-cell sarcoma patient-derived orthotopic xenograft (PDOX): precision-oncology nude-mouse model matching the patient with effective therapy.
J Cell Biochem. 2018 Aug;119(8):6598-6603.

Miwa S, Mochizuki T, Yamamoto N, Shirai T, Hayashi K, Takeuchi A, Inatani H, Igarashi K, Higuchi T, Abe K, Taniguchi Y, Aiba H, Ikeda H, Tsuchiya H.
Efficacy and Limitations of F-18-fluoro-2-deoxy-D-glucose Positron Emission Tomography to Differentiate Between Malignant and Benign Bone and Soft Tissue Tumors.
Anticancer Res. 2018 Jul;38(7):4065-4072.

Takeuchi A, Yamamoto N, Hayashi K, Matsubara H, Kimura H, Miwa S, Higuchi T, Abe K, Taniguchi Y, Tsuchiya H.
Growth of epiphysis after epiphyseal-preservation surgery for childhood osteosarcoma around the knee joint.
BMC Musculoskelet Disord. 2018 Jun 6;19(1):185.

Hayashi K, Yahata T, Muramoto R, Yamamoto N, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Aiba H, Araki Y, Tsuchiya H.
Factors Associated With Discharge Destination in Advanced Cancer Patients With Bone Metastasis in a Japanese Hospital.
Ann Rehabil Med. 2018 Jun 27;42(3):477-482.

Takeuchi A, Suwanpramote P, Yamamoto N, Shirai T, Hayashi K, Kimura H, Miwa S, Higuchi T, Abe K, Tsuchiya H.
Mid- to long-term clinical outcome of giant cell tumor of bone treated with calcium phosphate cement following thorough curettage and phenolization.
J Surg Oncol. 2018 May;117(6):1232-1238.

Higuchi T, Takeuchi A, Munesue S, Yamamoto N, Hayashi K, Kimura H, Miwa S, Inatani H, Shimozaki S, Kato T, Aoki Y, Abe K, Taniguchi Y, Aiba H, Murakami H, Harashima A, Yamamoto Y, Tsuchiya H.
Anti-tumor effects of a nonsteroidal anti-inflammatory drug zaltoprofen on chondrosarcoma via activating peroxisome proliferator-activated receptor gamma and suppressing matrix metalloproteinase-2 expression.
Cancer Med. 2018 May;7(5):1944-1954.

Higuchi T, Yamamoto N, Hayashi K, Takeuchi A, Kimura H, Miwa S, Igarashi K, Abe K, Taniguchi Y, Aiba H, Tsuchiya H.
Calcium Phosphate Cement in the Surgical Management of Benign Bone Tumors.
Anticancer Res. 2018 May;38(5):3031-3035.

Higuchi T, Yamamoto N, Shirai T, Hayashi K, Takeuchi A, Kimura H, Miwa S, Abe K, Taniguchi Y, Tsuchiya H.
Treatment outcomes of the simple bone cyst: A comparative study of 2 surgical techniques using artificial bone substitutes.
Medicine (Baltimore). 2018 May;97(18):e0572.

Igarashi K, Li S, Han Q, Tan Y, Kawaguchi K, Murakami T, Kiyuna T, Miyake K, Li Y, Nelson SD, Dry SM, Singh AS, Elliott IA, Russell TA, Eckardt MA, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM.
Growth of doxorubicin-resistant undifferentiated spindle-cell sarcoma PDOX is arrested by metabolic targeting with recombinant methioninase.
J Cell Biochem. 2018 Apr;119(4):3537-3544.

Aiba H, Yamada S, Mizutani J, Yamamoto N, Okamoto H, Hayashi K, Kimura H, Takeuchi A, Miwa S, Kawai A, Yoshimura K, Tsuchiya H, Otsuka T.
Clinical outcomes of radio-hyperthermo-chemotherapy for soft tissue sarcoma compared to a soft tissue sarcoma registry in Japan: a retrospective matched-pair cohort study.
Cancer Med. 2018 Apr;7(4):1560-1571.

El-Far AHAM, Munesue S, Harashima A, Sato A, Shindo M, Nakajima S, Inada M, Tanaka M, Takeuchi A, Tsuchiya H, Yamamoto H, Shaheen HME, El-Sayed YS, Kawano S, Tanuma SI, Yamamoto Y.
In vitro anticancer effects of a RAGE inhibitor discovered using a structure-based drug design system.
Oncol Lett. 2018 Apr;15(4):4627-4634.

Igarashi K, Kawaguchi K, Li S, Han Q, Tan Y, Gainor E, Kiyuna T, Miyake K, Miyake M, Higuchi T, Oshiro H, Singh AS, Eckardt MA, Nelson SD, Russell TA, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM.
Recombinant methioninase combined with doxorubicin (DOX) regresses a DOX-resistant synovial sarcoma in a patient-derived orthotopic xenograft (PDOX) mouse model.
Oncotarget. 2018 Apr 10;9(27):19263-19272.

Aiba H, Yamada S, Mizutani J, Yamamoto N, Okamoto H, Hayashi K, Kimura H, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Araki Y, Tsuchiya H, Otsuka T.
Preoperative evaluation of the efficacy of radio-hyperthermo-chemotherapy for soft tissue sarcoma in a case series.
PLoS One. 2018 Apr 16;13(4):e0195289.

Igarashi K, Kawaguchi K, Li S, Han Q, Tan Y, Murakami T, Kiyuna T, Miyake K, Miyake M, Singh AS, Eckardt MA, Nelson SD, Russell TA, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Singh SR, Eilber FC, Hoffman RM.
Recombinant methioninase in combination with doxorubicin (DOX) overcomes first-line DOX resistance in a patient-derived orthotopic xenograft nude-mouse model of undifferentiated spindle-cell sarcoma.
Cancer Lett. 2018 Mar 28;417:168-173.

Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Miyake M, Singh AS, Eckardt MA, Nelson SD, Russell TA, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Singh SR, Eilber FC, Hoffman RM.
Tumor-targeting Salmonella typhimurium A1-R is a highly effective general therapeutic for undifferentiated soft tissue sarcoma patient-derived orthotopic xenograft nude-mouse models.
Biochem Biophys Res Commun. 2018 Mar 18;497(4):1055-1061.

Higuchi T, Yamamoto N, Hayashi K, Takeuchi A, Abe K, Taniguchi Y, Araki Y, Tada K, Tsuchiya H.
Successful joint preservation of distal radius osteosarcoma by en bloc tumor excision and reconstruction using a tumor bearing frozen autograft: a case report.
BMC Surg. 2018 Mar 1;18(1):12.

Aiba H, Yamada S, Yamamoto N, Hayashi K, Miwa S, Tsuchiya H, Otsuka T.
Spontaneous shrinkage of solitary osteochondromas.
Skeletal Radiol. 2018 Jan;47(1):61-68.

Higuchi T, Yamamoto N, Hayashi K, Takeuchi A, Kato S, Miwa S, Abe K, Taniguchi Y, Aiba H, Tsuchiya H.
The Efficacy of Wide Resection for Musculoskeletal Metastatic Lesions of Renal Cell Carcinoma.
Anticancer Res. 2018 Jan;38(1):577-582.

Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Miyake M, Li S, Han Q, Tan Y, Zhao M, Li Y, Nelson SD, Dry SM, Singh AS, Elliott IA, Russell TA, Eckardt MA, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM.
Tumor-targeting Salmonella typhimurium A1-R combined with recombinant methioninase and cisplatinum eradicates an osteosarcoma cisplatinum-resistant lung metastasis in a patient-derived orthotopic xenograft (PDOX) mouse model: decoy, trap and kill chemotherapy moves toward the clinic.
Cell Cycle. 2018;17(6):801-809.

Aiba H, Yamada S, Yamamoto N, Hayashi K, Miwa S, Tsuchiya H, Otsuka T.
Spontaneous shrinkage of solitary osteochondromas.
Skeletal Radiol. 2018 Jan;47(1):61-68.

2017年

Yamamoto N, Tsuchiya H.
Biological Reconstruction Using Liquid Nitrogen-Treated Tumor Bearing Bone.
Osteosarcoma Biology, Behavior and Mechanisms. Kanya Honoki, Kurt Richard Weiss, ed. 1st ed. INTECH Zagreb 2017.

Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Miyake M, Li Y, Nelson SD, Dry SM, Singh AS, Elliott IA, Russell TA, Eckardt MA, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM.
Temozolomide combined with irinotecan regresses a cisplatinum-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) precision-oncology mouse model.
Oncotarget. 2017 Dec 4;9(8):7774-7781.

Miwa S, Shirai T, Yamamoto N, Hayashi K, Takeuchi A, Tada K, Kajino Y, Inatani H, Higuchi T, Abe K, Taniguchi Y, Tsuchiya H.
Risk factors for postoperative deep infection in bone tumors.
PLoS One. 2017 Nov 9;12(11):e0187438.

Higuchi T, Yamamoto N, Nishida H, Hayashi K, Takeuchi A, Kimura H, Miwa S, Inatani H, Shimozaki S, Kato T, Aoki Y, Abe K, Taniguchi Y, Tsuchiya H.
Knee joint preservation surgery in osteosarcoma using tumour-bearing bone treated with liquid nitrogen.
Int Orthop. 2017 Oct;41(10):2189-2197.

Igarashi K, Kawaguchi K, Murakami T, Kiyuna T, Miyake K, Singh AS, Nelson SD, Dry SM, Li Y, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM.
High Efficacy of Pazopanib on an Undifferentiated Spindle-Cell Sarcoma Resistant to First-Line Therapy Is Identified With a Patient-Derived Orthotopic Xenograft (PDOX) Nude Mouse Model.
J Cell Biochem. 2017 Sep;118(9):2739-2743.

Igarashi K, Kawaguchi K, Kiyuna T, Miyake K, Murakami T, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Hoffman RM.
Effective Metabolic Targeting of Human Osteosarcoma Cells In Vitro and in Orthotopic Nude-mouse Models with Recombinant Methioninase.
Anticancer Res. 2017 Sep;37(9):4807-4812.

Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Miwa S, Nelson SD, Dry SM, Li Y, Singh AS, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Eilber FC, Hoffman RM.
Efficacy In Vitro of Caffeine and Valproic Acid on Patient-Derived Undifferentiated Pleomorphic Sarcoma and Rhabdomyosarcoma Cell Lines.
Anticancer Res. 2017 Aug;37(8):4081-4084.

Zekry KM, Yamamoto N, Hayashi K, Takeuchi A, Higuchi T, Abe K, Taniguchi Y, Alkhooly AZAA, Abd-Elfattah AS, Fouly EH, Ahmed AR, Tsuchiya H.
Intercalary frozen autograft for reconstruction of malignant bone and soft tissue tumours.
Int Orthop. 2017 Jul;41(7):1481-1487.

Inatani H, Yamamoto N, Hayashi K, Kimura H, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Yamada S, Asai K, Otsuka T, Tsuchiya H.
Do Mesenchymal Stem Cells Derived From Atypical Lipomatous Tumors Have Greater Differentiation Potency Than Cells From Normal Adipose Tissues?
Clin Orthop Relat Res. 2017 Jun;475(6):1693-1701.

Igarashi K, Kawaguchi K, Murakami T, Kiyuna T, Miyake K, Nelson SD, Dry SM, Li Y, Yanagawa J, Russell TA, Singh AS, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Eilber FC, Hoffman RM.
Intra-arterial administration of tumor-targeting Salmonella typhimurium A1-R regresses a cisplatin-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) mouse model.
Cell Cycle. 2017 Jun 18;16(12):1164-1170.

Igarashi K, Kawaguchi K, Murakami T, Kiyuna T, Miyake K, Yamamoto N, Hayashi K, Kimura H, Nelson SD, Dry SM, Li Y, Singh AS, Miwa S, Odani A, Eilber FC, Tsuchiya H, Hoffman RM.
A novel anionic-phosphate-platinum complex effectively targets an undifferentiated pleomorphic sarcoma better than cisplatinum and doxorubicin in a patient-derived orthotopic xenograft (PDOX).
Oncotarget. 2017 Jun 28;8(38):63353-63359.

Rosario MS, Yamamoto N, Hayashi K, Takeuchi A, Kimura H, Miwa S, Higuchi T, Inatani H, Abe K, Taniguchi Y, Aiba H, Tsuchiya H.
An unusual case of proximal humeral simple bone cyst in an adult from secondary cystic change.
World J Surg Oncol. 2017 May 15;15(1):102.

Miwa S, Nishida H, Tanzawa Y, Takeuchi A, Hayashi K, Yamamoto N, Mizukoshi E, Nakamoto Y, Kaneko S, Tsuchiya H.
Phase 1/2 study of immunotherapy with dendritic cells pulsed with autologous tumor lysate in patients with refractory bone and soft tissue sarcoma.
Cancer. 2017 May 1;123(9):1576-1584.

Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Yamamoto N, Hayashi K, Kimura H, Miwa S, Tsuchiya H, Hoffman RM.
Antimetastatic Efficacy of the Combination of Caffeine and Valproic Acid on an Orthotopic Human Osteosarcoma Cell Line Model in Nude Mice.
Anticancer Res. 2017 Mar;37(3):1005-1011.

Igarashi K, Kawaguchi K, Kiyuna T, Murakami T, Miwa S, Nelson SD, Dry SM, Li Y, Singh AS, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Eilber FC, Hoffman RM.
Temozolomide combined with irinotecan caused regression in an adult pleomorphic rhabdomyosarcoma patient-derived orthotopic xenograft (PDOX) nude-mouse model.
Oncotarget. 2017 Mar 24;8(44):75874-75880.

Rosario M, Takeuchi A, Yamamoto N, Hayashi K, Miwa S, Higuchi T, Abe K, Taniguchi Y, Aiba H, Tanzawa Y, Murakami H, Tsuchiya H.
Pathogenesis of Osteosclerotic Change Following Treatment with an Antibody Against RANKL for Giant Cell Tumour of the Bone.
Anticancer Res. 2017 Feb;37(2):749-754.

Hayashi K, Yamamoto N, Shirai T, Takeuchi A, Kimura H, Miwa S, Higuchi T, Abe K, Taniguchi Y, Aiba H, Kiyohara H, Imai R, Ikeda H, Tsuchiya H.
Sequential histological findings and clinical response after carbon ion radiotherapy for unresectable sarcoma.
Clin Transl Radiat Oncol. 2017 Feb 6;2:41-45.

2016年

Rosario MS, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Inatani H, Higuchi T, Tsuchiya H.
A case of infected schwannoma mimicking malignant tumor.
World J Surg Oncol. 2016 Dec 7;14(1):302.

Kimura H, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Takeuchi A, Miwa S, Tsuchiya H.
Clinical Outcome of Reconstruction Using Frozen Autograft for a Humeral Bone Tumor.
Anticancer Res. 2016 Dec;36(12):6631-6635.

Miwa S, Toneri M, Igarashi K, Yano S, Kimura H, Hayashi K, Yamamoto N, Tsuchiya H, Hoffman RM.
Real-Time In Vivo Confocal Fluorescence Imaging of Prostate Cancer Bone-Marrow Micrometastasis Development at the Cellular Level in Nude Mice.
J Cell Biochem. 2016 Nov;117(11):2533-7.

Shimozaki S, Yamamoto N, Domoto T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Miwa S, Igarashi K, Kato T, Aoki Y, Higuchi T, Hirose M, Hoffman RM, Minamoto T, Tsuchiya H.
Efficacy of glycogen synthase kinase-3β targeting against osteosarcoma via activation of β-catenin.
Oncotarget. 2016 Nov 22;7(47):77038-77051.

Hayashi K, Niu X, Tang X, Singh VA, Asavamongkolkul A, Kawai A, Yamamoto N, Shirai T, Takeuchi A, Kimura H, Miwa S, Tsuchiya H.
Experience of total scapular excision for musculoskeletal tumor and reconstruction in eastern Asian countries.
J Bone Oncol. 2016 Oct 15;9:55-58.

Abdelaal AH, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Morsy AF, Kajino Y, Tsuchiya H.
The linea aspera as a guide for femoral rotation after tumor resection: is it directly posterior? A technical note.
J Orthop Traumatol. 2016 Sep;17(3):255-9.

Shimozaki S, Inatani H, Numata H, Tsuchiya H.
A Novel Technique for Calcaneal Avulsion Fracture: A Case Report.
J Trauma Treatment. 2016 5:4.

Takeuchi A, Yamamoto N, Shirai T, Hayashi K, Miwa S, Munesue S, Yamamoto Y, Tsuchiya H.
Clinical relevance of peroxisome proliferator-activated receptor-gamma expression in myxoid liposarcoma.
BMC Cancer. 2016 Jul 11;16:442.

Takeuchi A, Tsuchiya H, Ishii T, Nishida Y, Abe S, Matsumine A, Kawai A, Yoshimura K, Ueda T.
Clinical outcome of recurrent giant cell tumor of the extremity in the era before molecular target therapy: the Japanese Musculoskeletal Oncology Group study.
BMC Musculoskelet Disord. 2016 Jul 22;17:306.

Inatani H, Yamamoto N, Hayashi K, Kimura H, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Yamada S, Okamoto H, Otsuka T, Tsuchiya H.
Surgical management of proximal fibular tumors: A report of 12 cases.
J Bone Oncol. 2016 Jun 8;5(4):163-166.

Abe K, Yamamoto N, Hayashi K, Takeuchi A, Miwa S, Inatani H, Higuchi T, Taniguchi Y, Tsuchiya H.
Balancing Prolonged Survival with QoL Using Low-dose Pazopanib Maintenance: A Comparison with the PALETTE Study.
Anticancer Res. 2016 Jun;36(6):2893-7.

Miwa S, De Magalhães N, Toneri M, Zhang Y, Cao W, Bouvet M, Tsuchiya H, Hoffman RM.
Fluorescence-guided surgery of human prostate cancer experimental bone metastasis in nude mice using anti-CEA DyLight 650 for tumor illumination.
J Orthop Res. 2016 Apr;34(4):559-65.

Takeuchi A, Yamamoto N, Hayashi K, Miwa S, Takahira M, Fukui K, Oikawa T, Tsuchiya H.
Tenosynovial giant cell tumors in unusual locations detected by positron emission tomography imaging confused with malignant tumors: report of two cases.
BMC Musculoskelet Disord. 2016 Apr 26;17:180.

Abdelaal AH, Yamamoto N, Hayashi K, Takeuchi A, Morsy AF, Miwa S, Kajino Y, Rubio DA, Tsuchiya H.
Radiological assessment of the femoral bowing in Japanese population.
SICOT J. 2016 Jan 22;2:2.

2015年

Miwa S, Yano S, Kimura H, Yamamoto M, Toneri M, Murakami T, Hayashi K, Yamamoto N, Fujiwara T, Tsuchiya H, Hoffman RM.
Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging.
Cell Cycle. 2015;14(12):1932-7.

Miwa S, Yano S, Kimura H, Yamamoto M, Toneri M, Matsumoto Y, Uehara F, Hiroshima Y, Murakami T, Hayashi K, Yamamoto N, Bouvet M, Fujiwara T, Tsuchiya H, Hoffman RM.
Cell-cycle fate-monitoring distinguishes individual chemosensitive and chemoresistant cancer cells in drug-treated heterogeneous populations demonstrated by real-time FUCCI imaging.
Cell Cycle. 2015;14(4):621-9.

Takeuchi A, Yamamoto N, Shirai T, Nishida H, Hayashi K, Watanabe K, Miwa S, Tsuchiya H.
Successful correction of tibial bone deformity through multiple surgical procedures, liquid nitrogen-pretreated bone tumor autograft, three-dimensional external fixation, and internal fixation in a patient with primary osteosarcoma: a case report.
BMC Surg. 2015 Dec 7;15:124.

Abe K, Takeuchi A, Yamamoto N, Hayashi K, Tada K, Miwa S, Inatani H, Aoki Y, Higuchi T, Tsuchiya H.
Symptomatic small schwannoma is a risk factor for surgical complications and correlates with difficulty of enucleation.
Springerplus. 2015 Dec 1;4:751.

Subhadrabandhu S, Takeuchi A, Yamamoto N, Shirai T, Nishida H, Hayashi K, Miwa S, Tsuchiya H.
Frozen Autograft-Prosthesis Composite Reconstruction in Malignant Bone Tumors.
Orthopedics. 2015 Oct;38(10):e911-8.

Miwa S, Yokogawa A, Kobayashi T, Nishimura T, Igarashi K, Inatani H, Tsuchiya H.
Risk factors of recurrent lumbar disk herniation: a single center study and review of the literature.
J Spinal Disord Tech. 2015 Jun;28(5):E265-9.

Miwa S, Yano S, Yamamoto M, Matsumoto Y, Uehara F, Hiroshima Y, Toneri M, Murakami T, Kimura H, Hayashi K, Yamamoto N, Efimova EV, Tsuchiya H, Hoffman RM.
Real-time fluorescence imaging of the DNA damage repair response during mitosis.
J Cell Biochem. 2015 Apr;116(4):661-6.

Kimura H, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kato T, Aoki Y, Higuchi T, Tsuchiya H.
Efficacy of triplet regimen antiemetic therapy for chemotherapy-induced nausea and vomiting (CINV) in bone and soft tissue sarcoma patients receiving highly emetogenic chemotherapy, and an efficacy comparison of single-shot palonosetron and consecutive-day granisetron for CINV in a randomized, single-blinded crossover study.
Cancer Med. 2015 Mar;4(3):333-41.

Hayashi K, Yamada S, Inatani H, Okamoto H, Takeuchi A, Nishida H, Yamamoto N, Tsuchiya H, Otsuka T.
Salvage method for unplanned excision of soft tissue sarcoma: long-term results of second-look surgery following radio-hyperthermo-chemotherapy.
Anticancer Res. 2015 Jan;35(1):493-8.

2014年

Igarashi K, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Miwa S, Inatani H, Shimozaki S, Kato T, Tsuchiya H.
Late recurrence of osteosarcoma: a report of two cases.
J Orthop Surg (Hong Kong). 2014 Dec;22(3):415-9.

Miwa S, Zhang Y, Baek KE, Uehara F, Yano S, Yamamoto M, Hiroshima Y, Matsumoto Y, Kimura H, Hayashi K, Yamamoto N, Bouvet M, Tsuchiya H, Hoffman RM, Zhao M.
Inhibition of spontaneous and experimental lung metastasis of soft-tissue sarcoma by tumor-targeting Salmonella typhimurium A1-R.
Oncotarget. 2014 Dec 30;5(24):12849-61.

Miwa S, Hiroshima Y, Yano S, Zhang Y, Matsumoto Y, Uehara F, Yamamoto M, Kimura H, Hayashi K, Bouvet M, Tsuchiya H, Hoffman RM.
Fluorescence-guided surgery improves outcome in an orthotopic osteosarcoma nude-mouse model.
J Orthop Res. 2014 Dec;32(12):1596-601.

Miwa S, Matsumoto Y, Hiroshima Y, Yano S, Uehara F, Yamamoto M, Zhang Y, Kimura H, Hayashi K, Yamamoto N, Bouvet M, Sugimoto N, Tsuchiya H, Hoffman RM.
Fluorescence-guided surgery of prostate cancer bone metastasis.
J Surg Res. 2014 Nov;192(1):124-33.

Takeuchi A, Lewis VO, Satcher RL, Moon BS, Lin PP.
What are the factors that affect survival and relapse after local recurrence of osteosarcoma?
Clin Orthop Relat Res. 2014 Oct;472(10):3188-95.

Miwa S, Takeuchi A, Shirai T, Yamamoto N, Hayashi K, Nishida H, Kimura H, Igarashi K, Tsuchiya H.
Outcomes and complications of reconstruction using tumor-bearing frozen autografts in patients with metastatic bone tumors.
Anticancer Res. 2014 Oct;34(10):5569-77.

Miwa S, Yano S, Zhang Y, Matsumoto Y, Uehara F, Yamamoto M, Hiroshima Y, Kimura H, Hayashi K, Yamamoto N, Bouvet M, Tsuchiya H, Hoffman RM, Zhao M.
Tumor-targeting Salmonella typhimurium A1-R prevents experimental human breast cancer bone metastasis in nude mice.
Oncotarget. 2014 Aug 30;5(16):7119-25.

Hayashi K, Iwata S, Ogose A, Kawai A, Ueda T, Otsuka T, Tsuchiya H.
Factors that influence functional outcome after total or subtotal scapulectomy: Japanese Musculoskeletal Oncology Group (JMOG) study.
PLoS One. 2014 Jun 17;9(6):e100119.

Kimura H, Yamamoto N, Nishida H, Hayashi K, Takeuchi A, Nojima T, Ikeda H, Sawada-Kitamura S, Tsuchiya H.
Synovial sarcoma in knee joint, mimicking low-grade sarcoma confirmed by molecular detection of SYT gene split.
Anticancer Res. 2014 Jun;34(6):3105-11.

Igarashi K, Yamamoto N, Shirai T, Hayashi K, Nishida H, Kimura H, Takeuchi A, Tsuchiya H.
The long-term outcome following the use of frozen autograft treated with liquid nitrogen in the management of bone and soft-tissue sarcomas.
Bone Joint J. 2014 Apr;96-B(4):555-61.

Shimozaki S, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Kato T, Tsuchiya H.
Pedicle versus free frozen autograft for reconstruction in malignant bone and soft tissue tumors of the lower extremities.
J Orthop Sci. 2014 Jan;19(1):156-63.

2013年

Yamamoto N, Tsuchiya H.
Chemotherapy for osteosarcoma – where does it come from? What is it? Where is it going?
Expert Opin Pharmacother. 2013 Nov;14(16):2183-93.

Takeuchi A, Yamamoto N, Nishida H, Kimura H, Ikeda H, Tsuchiya H.
Complete necrosis of a giant cell tumor with high expression of PPARγ: a case report.
Anticancer Res. 2013 May;33(5):2169-74.

Takeuchi A, Yamamoto Y, Munesue S, Harashima A, Watanabe T, Yonekura H, Yamamoto H, Tsuchiya H.
Low molecular weight heparin suppresses receptor for advanced glycation end products-mediated expression of malignant phenotype in human fibrosarcoma cells.
Cancer Sci. 2013 Jun;104(6):740-9.

Kimura H, Dobashi Y, Nojima T, Nakamura H, Yamamoto N, Tsuchiya H, Ikeda H, Sawada-Kitamura S, Oyama T, Ooi A.
Utility of fluorescence in situ hybridization to detect MDM2 amplification in liposarcomas and their morphological mimics.
Int J Clin Exp Pathol. 2013 Jun 15;6(7):1306-16.

2012年

Yamamoto N, Hayashi K, Yamauchi K, Takeuchi A, Kimura H, Igarashi K, Hoffman RM, Tsuchiya H.
In vitro and in vivo models for real-time or time course tumor cell imaging.
Int J Mol Med. 2012 30: S6.

Yamamoto N, Inatani H, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Shimozaki S, Kato T, Tsuchiya H.
Histological study on liquid nitrogen-treated tumor-bearing bone of the proximal femur excised eight years after transplantation.
Low Temp Med. 2012 38: 5-8.

Kimura H, Tome Y, Momiyama M, Hayashi K, Tsuchiya H, Bouvet M, Hoffman RM.
Imaging the inhibition by anti-β1 integrin antibody of lung seeding of single osteosarcoma cells in live mice.
Int J Cancer. 2012 Nov 1;131(9):2027-33.

Tsuchiya H, Shirai T, Nishida H, Murakami H, Kabata T, Yamamoto N, Watanabe K, Nakase J.
Innovative antimicrobial coating of titanium implants with iodine.
J Orthop Sci. 2012 Sep;17(5):595-604.

Miwa S, Taki J, Yamamoto N, Shirai T, Nishida H, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Ooi A, Tsuchiya H.
A novel combined radiological method for evaluation of the response to chemotherapy for primary bone sarcoma.
J Surg Oncol. 2012 Sep 1;106(3):273-9.

Miwa S, Sugimoto N, Yamamoto N, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Yachie A,
Tsuchiya H. Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways.
Anticancer Res. 2012 Sep;32(9):3643-9.

Yamamoto N, Hayashi K, Tanzawa Y, Kimura H, Takeuchi A, Igarashi K, Inatani H, Shimozaki S, Kitamura S, Tsuchiya H.
Treatment strategies for well-differentiated liposarcomas and therapeutic outcomes.
Anticancer Res. 2012 May;32(5):1821-5.

Miwa S, Nishida H, Tanzawa Y, Takata M, Takeuchi A, Yamamoto N, Shirai T, Hayashi K, Kimura H, Igarashi K, Mizukoshi E, Nakamoto Y, Kaneko S, Tsuchiya H.
TNF-α and tumor lysate promote the maturation of dendritic cells for immunotherapy for advanced malignant bone and soft tissue tumors.
PLoS One. 2012;7(12):e52926.

2011年

Hayashi K, Karita M, Yamamoto N, Shirai T, Nishida H, Takeuchi A, Kimura H, Miwa S, Tsuchiya H.
Functional outcomes after total scapulectomy for malignant bone or soft tissue tumors in the shoulder girdle.
Int J Clin Oncol. 2011 Oct;16(5):568-73.

Miwa S, Shirai T, Taki J, Sumiya H, Nishida H, Hayashi K, Takeuchi A, Ooi A, Tsuchiya H.
Use of 99mTc-MIBI scintigraphy in the evaluation of the response to chemotherapy for osteosarcoma: comparison with 201Tl scintigraphy and angiography.
Int J Clin Oncol. 2011 Aug;16(4):373-8.

Tsuchiya H, Takeuchi A, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tomita K.
High-grade undifferentiated pleomorphic sarcoma of pelvis treated with curettage and bone graft after complete remission following caffeine-potentiated chemotherapy.
J Orthop Sci. 2011 Jul;16(4):476-81.

Nishida H, Yamamoto N, Tanzawa Y, Tsuchiya H.
Cryoimmunology for malignant bone and soft-tissue tumors.
Int J Clin Oncol. 2011 Apr;16(2):109-17.

Tsuchiya H.
Basic science and clinical frontiers of sarcoma treatment.
Int J Clin Oncol. 2011 Apr;16(2):82-3.

Yamamoto N, Tsuchiya H, Hoffman RM.
Tumor imaging with multicolor fluorescent protein expression.
Int J Clin Oncol. 2011 Apr;16(2):84-91.

Takeuchi A, Tsuchiya H, Niu X, Ueda T, Jeon DG, Wang EH, Asavamongkolkul A, Kusuzaki K, Sakayama K, Kang YK.
The prognostic factors of recurrent GCT: a cooperative study by the Eastern Asian Musculoskeletal Oncology Group.
J Orthop Sci. 2011 Mar;16(2):196-202.

Tanzawa Y, Tsuchiya H, Shirai T, Nishida H, Hayashi K, Takeuchi A, Kawahara M, Tomita K.
Potentiation of the antitumor effect of calcium phosphate cement containing anticancer drug and caffeine on rat osteosarcoma.
J Orthop Sci. 2011 Jan;16(1):77-84.

2010年

Kimura H, Momiyama M, Tomita K, Tsuchiya H, Hoffman RM.
Long-working-distance fluorescence microscope with high-numerical-aperture objectives for variable-magnification imaging in live mice from macro- to subcellular.
J Biomed Opt. 2010 Nov-Dec;15(6):066029.

Miwa S, Kitamura S, Shirai T, Hayashi K, Nishida H, Takeuchi A, Nojima T, Tsuchiya H.
Desmoplastic small round cell tumour successfully treated with caffeine-assisted chemotherapy: a case report and review of the literature.
Anticancer Res. 2010 Sep;30(9):3769-74.

Kimura H, Lee C, Hayashi K, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Bouvet M, Hoffman RM.
UV light killing efficacy of fluorescent protein-expressing cancer cells in vitro and in vivo.
J Cell Biochem. 2010 Aug 15;110(6):1439-46.

Tsuchiya H, Nishida H, Srisawat P, Shirai T, Hayashi K, Takeuchi A, Yamamoto N, Tomita K.
Pedicle frozen autograft reconstruction in malignant bone tumors.
J Orthop Sci. 2010 May;15(3):340-9.

Kawano M, Nishida H, Nakamoto Y, Tsumura H, Tsuchiya H.
Cryoimmunologic antitumor effects enhanced by dendritic cells in osteosarcoma.
Clin Orthop Relat Res. 2010 May;468(5):1373-83.

Kimura H, Hayashi K, Yamauchi K, Yamamoto N, Tsuchiya H, Tomita K, Kishimoto H, Bouvet M, Hoffman RM.
Real-time imaging of single cancer-cell dynamics of lung metastasis.
J Cell Biochem. 2010 Jan 1;109(1):58-64.